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KMID : 0380020150300020053
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 2 p.53 ~ p.57
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Optimum Reaction Condition of Enzymatic Hydrolysis for Production of Reducing Sugar from Enteromorpha intestinalis
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Kim A-Ram
Kim Dong-Hyun
Jeong Gwi-Taek
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Abstract
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In this study, the production of total reducing sugar from macro green-algae Enteromorpha intestinalis by enzymatic hydrolysis was investigated. As a result of enzymatic hydrolysis using 13 kind commercial enzymes, the highest yield of 8.75% was obtained from Viscozyme L, which is multi-enzyme complex such as cellulase, arabanase, beta-glucanase, hemicellulase and xylanase. As a control, only 0.33% and 0.27% yield were obtained from 1% sulfuric acid and 0.05 M citrate buffer (pH 4.8), respectively. In the case of enzyme mixture, the mixture of Viscozyme® L and Cellic® CTec2 (1:1) was presented the highest yield of 10.67%. Finally, the 14.99% yield was obtained at 36 hr under the condition of 10% biomass and 30% enzyme mixture.
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KEYWORD
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Enteromorpha intestinalis, Enzymatic hydrolysis, Reducing sugar
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